33P How does it work?

tflanigan at molbiol.ox.ac.uk tflanigan at molbiol.ox.ac.uk
Fri Apr 21 06:23:13 EST 1995


In article <D7CLos.5Dw at ncifcrf.gov>, pnh at fcs260c2.ncifcrf.gov (Paul N Hengen) writes:
>  In article <1995Apr19.182404.11700 at emba.uvm.edu>
>  kbarthol at moose.uvm.edu (Kirk Bartholomew) writes:
> 
> | I've seen a number of advertisments for 33P nucleotides in the 
> | past few months. Is anybody using this stuff?  Does it work as well as 
> | 35S.  Post to this news group and I will compile a summary.
> 
> There was a flurry of discussion last year about the use of 33-P for
> sequencing, if that is what you are interested in. I suggest reading
> the review that I wrote [as always]. Is it that nobody knows about the
> monthly column that covers these kinds of topics??? Of course, I'd love
> to see anything _new_ posted about using 33-P.  I've thought about
> posting the list of topics already covered, but I can already hear groans
> coming over the net. Okay then, look at my ftp site, or read the FAQ list.
> 
> @article{Hengen1994Maytibs,
> author = "P. N. Hengen",
> title = "Methods and reagents - Use of {${}^{33}{\mbox{P}}$}:
> a safer way to sequence?",
> journal = "Trends in Biochemical Sciences",
> volume = "19",
> number = "5",
> pages = "227-229",
> month = "may",
> year = "1994"}
> 
> BTW, if you didn't recognize it, {${}^{33}{\mbox{P}}$} means 33-P.
> 
> *******************************************************************************
> * Paul N. Hengen, Ph.D.                           /--------------------------/*
> * National Cancer Institute                       |Internet: pnh at ncifcrf.gov |*
> * Laboratory of Mathematical Biology              |   Phone: (301) 846-5581  |*
> * Frederick Cancer Research and Development Center|     FAX: (301) 846-5598  |*
> * Frederick, Maryland 21702-1201 USA              /--------------------------/*
> * - - - - - Methods FAQ list -> http://www-lmmb.ncifcrf.gov/~pnh/ - - - - - - *
> * - - -  Anonymous FTP from ftp.ncifcrf.gov as file pub/methods/FAQlist - - - *
> *******************************************************************************
Reply to  .. 33P how does it work?
Peng Liang wrote an article in BIOTECHNIQUES recently on the use of 33P for 
differential display as a replacement for 35S.  The data presented looked good
and  .. yes it did work!



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