Preparation of ribosomes or ribosomal subunits from blood?

Stephan Witte stephan.witte at uni-konstanz.de
Fri Apr 21 09:25:39 EST 1995


Hi,
I«m working on the characterization of some human ribosomal proteins of the
large subunit. Up to know i was workong with recombinant proteins,
expressed in e. coli or in the baculovirus system.
Now i want to proof, that those recombinant proteins are good models for
the native protein.
I«ve heard that it is possible to prepare human ribosomes from blood. Has
anyone a protocol and/or reference?
Is it poosible to prepare amounts of 200-300 ug per ribosomal protein?

Stephan Witte
Inst. of Immunology
University of Constance
stephan.witte at uni-konstanz.de



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