Transfection into Adipocytes

Simon Hui shui at biosci.cbs.umn.edu
Fri Apr 21 12:40:17 EST 1995


Dear netters,

I would like to ask if any of you have a good protocol for tranfecting DNA
into cultured adipocytes.  I work with 3T3-L1 cells and have tried both
calcium phosphate method and electroporation (0.4cm cuvette, 650V, 25uF);
however, both methods didn't give good transfection efficiency.  

Thanks in advance.

Simon Hui
Department of Biochemistry
University of Minnesota, Twin Cities
Internet: shui at biosci.cbs.umn.edu




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