Quick Denature Plasmid Sequencing Kit

Steven Goldberg goldberg at bms.com
Mon Apr 24 07:37:03 EST 1995


Has anyone had experience using USB/Amersham's new Quick Denature 
Plasmid Sequencing Kit?  It uses NaOH denaturation followed by HCl
renaturation prior to sequencing.  I have been trying it with high
G + C templates and have been getting rather weak sequences with a
lot of stops (I am including deaza-dGTP), more so than with the 
"standard" heat denaturation.  Any feedback would be appreciated.

Thanks. 

Steve Goldberg



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