Getting the GST out of pGEX?
crasmussen at anat.med.ualberta.ca
Tue Apr 25 12:04:23 EST 1995
kowalski at unixg.ubc.ca (Paul Edward Kowalski) wrote:
>I'd like to use the Tac promoter in pGEX2T to drive expression of a
>non-GST-fused protein: is there a restriction site (s) that allows the
>removal of the 26kD GST ORF from pGEX? There's nothing obvious in the
>original pGEX paper or some subsequent modifications papers. Thanks-
>UBC, Medical Genetics
There are other expression plasmids available that use the Tac promoter
like pKK223-3 from Pharmacia.
University of Alberta
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