Getting the GST out of pGEX?

Colin Rasmussen crasmussen at anat.med.ualberta.ca
Tue Apr 25 12:04:23 EST 1995



kowalski at unixg.ubc.ca (Paul Edward Kowalski) wrote:
>I'd like to use the Tac promoter in pGEX2T to drive expression of a 
>non-GST-fused protein: is there a restriction site (s) that allows the 
>removal of the 26kD GST ORF from pGEX? There's nothing obvious in the 
>original pGEX paper or some subsequent modifications papers. Thanks-
>
>
>Paul Kowalski
>UBC, Medical Genetics

There are other expression plasmids available that use the Tac promoter 
like pKK223-3 from Pharmacia.

Colin Rasmussen
University of Alberta
>
>





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