HELP-polyacrylamide gels cracking

ANDREI POPOV ANDREI.POPOV at bbsrc.ac.uk
Tue Apr 25 11:57:41 EST 1995


Subject: HELP-polyacrylamide gels cracking
>
> acrylamide (with 7 M urea) gels that are 0.8 mm
> thick (twice the thickness of my sequencing gels). Each time I have tried to
> dry them they crack like shattered glass!!!!
>
> Someone out there must know the trick to drying high percentage gels that
> are 0.8 mm thick.   Help  Help
>
> I have tried drying at 50 or 60 C, but the gels take forever to dry and
still > crack.
                
Try to soak in 10-15% Glycerol.
Another way is to dry on 3MM paper.




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