Getting the GST out of pGEX?

Paul Edward Kowalski kowalski at
Tue Apr 25 11:56:49 EST 1995

I'd like to use the Tac promoter in pGEX2T to drive expression of a 
non-GST-fused protein: is there a restriction site (s) that allows the 
removal of the 26kD GST ORF from pGEX? There's nothing obvious in the 
original pGEX paper or some subsequent modifications papers. Thanks-

Paul Kowalski
UBC, Medical Genetics

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