R.E. sites in Vectors

Stuart Kuhstoss sk at lilly.com
Tue Apr 25 11:37:40 EST 1995


In Article <cyost-2404950819560001 at chevron21.bio.ucalgary.ca>,
cyost at acs.ucalgary.ca (Chris Yost) wrote:
>This message may seem like bitching however I need to vent.  Is it not
>resonable to ask that when someone creates a useful vector construct that
>the include all of the restriction sites in the published paper. Ok, all
>the sites may be too much, especially exotic strange cutters.  However,  I
>think that at least all the sites that are in the M.C.S of pBluescript and
>puc derivatives should be included.  Especially if the construct that was
>made is a tool that can be used to make several new constructs.  
>
>(Stuff deleted)
>Chris Yost
>cyost at acs.ucalgary.ca

Let me make a suggestion.  If a site is important for a cloning experiment
(either its presence or absence), then confirm it by cutting the DNA
yourself.  That way you know what you're starting with.  Corrolary:  Don't
assume that a sequence of a vector is correct, either.  

Stu Kuhstoss                              



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