aaron at ccat.sas.upenn.edu
Tue Apr 25 10:56:16 EST 1995
Someone posted a message about 7M urea 0.8mm thick gels cracking during
I routinely dry 12M 0.8mm gels and have had no problems with drying. What
I usually do is this:
Seperate one plate from gel.
Put on a piece of Whatmann paper on tope of the gel and then slowly peel
up from one side. Place paper with gel on top onto drier, cover with
saran-wrap and flap down the plastic top of the drier.
I dry under a vacuum from a vacuum pump (not sure how strong) at 80C.
I dry the gel for 45-60 min, then remove vacuum and uncover the gel and
leave the heat on for about 5-10 minutes to remove any excess moisture.
Don't know if this helps. I've also read that you can make the gel with
5-15% glycerol to help eliminate cracking.
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