Degenerate Primers

Jeffrey Shane Temple jstemple at whale.st.usm.edu
Tue Apr 25 10:31:56 EST 1995


I just had two 18 base primers designed for use in PCR.  They are
degenerate in approximately 7 positions in that any nucleotide can pair.
I was wondering if anyone with more experience knows what concentration of
these primers to use in PCR?  It seems that you would use more than
non-degenerate primers, but I am not sure how much more. Any help would be
greatly appreciated.

Jeffrey Temple
Univ of Southern MS
Dept of Chem/Biochem



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