Degenerate Primers

Jeffrey Shane Temple jstemple at
Tue Apr 25 10:31:56 EST 1995

I just had two 18 base primers designed for use in PCR.  They are
degenerate in approximately 7 positions in that any nucleotide can pair.
I was wondering if anyone with more experience knows what concentration of
these primers to use in PCR?  It seems that you would use more than
non-degenerate primers, but I am not sure how much more. Any help would be
greatly appreciated.

Jeffrey Temple
Univ of Southern MS
Dept of Chem/Biochem

More information about the Methods mailing list