RNA isolation from plants

tatsuo kakimoto kakimoto at gen-info.osaka-u.ac.jp
Tue Apr 25 10:00:43 EST 1995


removal of polysaccharide
Disolve the RNA in TE, add 1/10 vol 3M NaOAc and set on ice for 20
min,then spin 30 min.  Most of the polysaccharides will precipitate,
while RNA will not precipitate.  Then ethanol precipitate.  Polysacchride
is successfully removed from RNA preparerd from Arabidopsis callus and
tobocco cultured cell(phenol-SDS method).
 Tatsuo Kakimoto



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