southern in roller bottle hybridizations
leach at bu.edu
Tue Apr 25 09:14:44 EST 1995
AMRUT at OITVMS.OIT.UMASS.EDU (AMRUT S BHOGLE) wrote:
>A friend of mine has been having problems doing Southern Hybridizations
>a roller bottle hybridization oven. The Nylon mesh seems to leave an
>on the membrane and wouldn't come off. The hybridization seems to be
>imperfect too I would appreciate if you have experienced such a problem
>if you could give me suggestions to avoid such a problem.
Could be due to dirty meshes...
I use polyester ones myself (silk-screening material from an art-shop)
and never have the problem. I have performed this type of hybridisation
with Southerns and library screening..always got a signal...
How do you determine whether it is imperfect??
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