How to improve transcfection efficiency ?
leach at bu.edu
Tue Apr 25 09:11:07 EST 1995
the simplest method I found was to decrease the media volume..so that
the concentration of DNA is higher!!
Using glycerol shock and lower culture volumes during transfection..my
promoter activity number jumped 8-10xfold
my promoter is pretty strong so I didn't need to go this high in the
Toshiharu Ishizuka <isizuka at med.m.chiba-u.ac.jp> wrote:
>We intend to transfect plasmids to mammalian cells. Does anybody know
some new drugs
>or treatment like chloquine or glycerol shock, to improve the
transfection efficiency ?
>We can use electroporation, lipofectoamine, and calcium phosphate
>Any comments are O. K. Please teach me.
>Department of Biochemistry,
>Chiba University, School of Medicine
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