GST-activity, binding buffer
you at somehost.somedomain
Tue Apr 25 08:15:49 EST 1995
Dear Netters; I was wondering if someone has tried systematically to check whether
the GST expressed in E.Coli (using the pGEX system) binds the GSH-column
under different buffers, detergents, salts etc conditions?.
i.e Does extraction in Hepes or
tris buffer allow similar binding of the GST to the column compared with PBS?
Does the GST activity remain the same in Hepes or tris buffers as
Thanks in advanced, Maor Bar-Peled.
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