GST-activity, binding buffer

Maor Bar-Peled you at somehost.somedomain
Tue Apr 25 08:15:49 EST 1995


Dear Netters; I was wondering if someone has tried systematically to check whether 
the GST expressed in E.Coli (using the pGEX system) binds the GSH-column
under different buffers, detergents, salts etc conditions?. 
 i.e Does extraction in Hepes or 
tris buffer allow similar binding of the GST to the column compared with PBS? 
             Does the GST activity remain the same in Hepes or tris buffers as
in PBS?
Thanks in advanced, Maor Bar-Peled.



More information about the Methods mailing list