aged chloroform inactivates lambda?

John E. Fox altabios at bham.ac.uk
Tue Apr 25 07:55:30 EST 1995


In article <jpcd0-2504951115450001 at macr1-2.welc.cam.ac.uk>, jpcd0 at mole.bio.cam.ac.uk (John Dixon) says:
>
>I just read in Maniatis (page 2.63) that aged batches of chloroform can
>inactivate lambda phage. Thinking back, this may explain a couple of
>recent low titres I have observed.
>


The problem comes from HCl which slowly builds up as the solvent
degrades. You could just wash your chloroform with water to 
remove the excess acid.

John Fox



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