purification of long PCR frags

Konstantine Sarafis Kara_Tech_Inc at mindlink.bc.ca
Wed Apr 26 22:33:36 EST 1995


In article <1995Apr24.020317.24733 at lugb.latrobe.edu.au>, Susanna Herd
<sherd at bioserver.latrobe.edu.au> wrote:

> Hi All,
>         Can any of you out there tell me the best way of 
> purifying long PCR fragments? I have a 15 KB amplified fragment
> that I want to purify.
>   Thanks for your help,
>     Cheers,
>            Sue

Try using NucleotrapCR from Macherey-Nagel, Duren, Germany. It is a silica
based system that utilizes chaotropic salts to remove the hydrate mantle
and allows to bind to the charged silica. 

Regards,

Konstantine



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