how to remove ATP from ADP?

DREWES at MPASMB.DESY.DE DREWES at MPASMB.DESY.DE
Wed Apr 26 17:19:01 EST 1995


You can obtain ATP-free ADP by column purification. For small amounts,
reverse phase HPLC is convenient. For 1 to 10 g ADP, dissolve in 0.1
M triethylammonium bicarbonate, load on anion exchange column (i.e.
QAE-Sephadex, 10-30 ml) and gradient elute with 0.1 - 0.3 M TEABC.
Lyophilize if desired. Store always in solution at pH 6.8, frozen. Will
slowly form AMP and ATP, approx. 0.1 % per month.
Hope this helps,

Gerard Drewes
MPG Struct. Biol., Hamburg, Germany.



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