Two Hybrid Problem

Robert Hindges hindges at vetbio.unizh.ch
Fri Apr 28 16:23:09 EST 1995


Hello
I am using pGBT9 for producing my protein as a fusion with the GAL4 BD. But
I can't detect any fusionprotein in westerns loading about 40ug of protein
extract on the gel. I sequenced of course the construct to proove in frame
cloning. Any suggestions of more experienced user of this system would be
appreciated. Thank you.



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