Oligo-ligation

[Martin Leach]

slawek at ALTIS.CHEM.PG.GDA.PL (Slawek Dabrowski) wrote:
>
>
>On Thu, 27 Apr 1995, Slawek Dabrowski wrote:
>
>> 
>> Hi,
>> 
>> 	I have problem with ligation an oligonucleotide to DNA fragment 
>> with cohesive termini. 
>> 
>> Does it will be work ? 
>> 
>> 
>>       5'-----------G  5'P------------------------3'
>>                      C---------------------------5'
>> 
>>                        |
>>                        | Ligation (T4 DNA ligase in standard buffer)
>>                       \ /
>>                      
>>       5'--------------G---------------------------3'
>>                       C---------------------------5'
>> 
>> 


I remember when performing a 5'RACE-like technique called SLIC that RNA 
ligase was used. I believe this can ligate oligos onto the end of 
DNA...cant remember if the DNA has to be single stranded though..

I believe Pharmacia sells RNA ligase..perhaps they could help you more.

ciao

Martin



-------------- next part --------------

....             Martin Leach                Email:leach at bu.edu 
   _|_______      Dept. of Pharmacology       Phone: (617) 638-5323        
   /  o    /      Boston Univ. School of Med. Fax:   (617) 638-4329         
 _/   |-  /__==/  80 E. Concord St. (L603)
(TURBODOZER) \_   Boston MA 02118            "Not the old underpants
                  USA                         on your head....WIBBLE
                                             " -BLACKADDER
My home page: http://155.41.115.114/Leach.html
Biotech Company WWW registry: http://155.41.115.114/jumper/biolcomm.html