DNA sequence needed: pBK-CMV
ladasky at leland.Stanford.EDU
Fri Apr 28 12:35:26 EST 1995
In article <3npukn$gv4 at nuscc.nus.sg>,
Chia Jin Ngee <mcblab47 at leonis.nus.sg> wrote:
>I need the DNA sequence of Stratagene's pBK-CMV plasmid. Can anyone direct
>me to any source on the net or anyone can email it to me.
A few months ago, I took the pages out of the Stratagene Zap Express
instruction booklet that have the pBK-CMV sequence, and scanned it in to a
computer. I haven't exhaustively proofread the resulting file, but I think
I cleaned it up pretty well (you know that OCR programs always make a few
mistakes). I compared the restriction fragment locations given by Strata-
gene to the results I got with a nucleic acid analysis program. I chose ten
enzymes at random, and my results matched Stratagene's table exactly.
If you want a copy of this file, email me. I can send it as plain
text or as a GenBank file.
Unique ID : Ladasky, John Joseph Jr.
Title : BA Biochemistry, U.C. Berkeley, 1989 (Ph.D. perhaps 1998???)
Location : Stanford University, Dept. of Structural Biology, Fairchild D-105
Keywords : immunology, music, running, Green
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