Purification of GST fusion proteins
gr8bn at cc.usu.edu
Sat Apr 29 12:53:06 EST 1995
In article <D7oywH.EoK at murdoch.acc.Virginia.EDU> "R. John Lye" <rjl6n at uva.pcmail.virginia.edu> writes:
>Relay-Version: ANU News - V6.1 08/24/93 VAX/VMS V6.1; site cc.usu.edu
>Subject: Re: Purification of GST fusion proteins
>Message-ID: <D7oywH.EoK at murdoch.acc.Virginia.EDU>
>From: "R. John Lye" <rjl6n at uva.pcmail.virginia.edu>
>Date: Thu, 27 Apr 1995 11:36:16 GMT
>Sender: usenet at murdoch.acc.Virginia.EDU
>References: <01HPT3KASBAQBIJ6L3 at MPGARS.DESY.DE>
>Organization: University of Virginia
>> Our GST fusion protein (approx. 100 kDa) does not bind to Pharmacia Glutathione Sepa
>> Sepharose under standard conditions.
>Is it soluble or is it going into inclusion bodies?
I also encountered the same kind of problem of my GST fusion protein,
and I was very sure thta it is soluble (confirmed by immunodetection).
The fusion protein binds to glutarthion-beads (Sigma) in a very low
efficiency manner, and most of the fusion proteins would be washed from
the beads with only on wash.
Gr8bn at cc.usu.edu
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