Hanahan competent E.coli

Colin Rasmussen crasmussen at anat.med.ualberta.ca
Sun Apr 30 21:12:52 EST 1995


My experience has been that for standard subcloning, the good old 100mM 
CaCl2 treatment is adequate to get the clones you want, and a lot 
simpler too.

Colin

p.s. I used to use the Hanahan procedure until I found out the Ca method 
was giving me all the transformants I needed.





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