Ferret Brain cDNA Library Mystery

Anthony Palombella palomb at beagle.Colorado.EDU
Sun Apr 30 08:49:49 EST 1995


Sorry for the off-topic comment, but I just have to say that "Ferret Brain
cDNA Library Mystery" is the best subject line I've ever read.

Anyway, as said before, secondary structure can be a real problem.  If you
try to make a new library, you might consider a reverse transcriptase with
increased thermostability.  I hear that Tth polymerase has RT activity at
70C.  Good luck.



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