Hanahan competent E.coli

Wises Namwat - SCMI - 3636183 gscwnw at mahidol.ac.th
Sat Apr 29 14:49:41 EST 1995


On Wed, 19 Apr 1995, Paul N Hengen wrote:
> ...  Also, make sure you read the
> articles below. Good luck catching the cells at this O.D. ;-)
> 
> @article{Tang1994,
> author = "X. Tang...
> title = "The optimization of preparations of 
competent > cells for transformation of {{\em E. coli}}",
> journal = "Nucl. Acids Res.",
> volume = "22",
> number = "14",
> pages = "2857-2858",
> comment = "1x10^8 transformants per ug DNA at O.D. 0.94",
> year = "1994"}
> 
	From my experiment in last two mont, DH5alpha at O.D.600nm 0.94 
provide me two times better transformation efficiency to that of O.D. 
0.60, by the Hanahan method. 
----------------------------------------------------------
Wises Namwat             E-mail: gscwnw at mucc.mahidol.ac.th
Dep. Microbiology,       Tel.66-02-2461358-74 Ext.4611    
Fac. Science,
Mahidol University,
Bangkok, Thailand.
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