seemingly two bands of one band in SDS-PAGE?
paiks at ari.net
Tue Aug 1 01:02:10 EST 1995
>Separation of protein bands of higher M.W ( 60 kDa) is
>ok, however that of lower one (25 kDa) is strange sometimes
>in that it has two forms of bands rather than one in
>SDS-PAGE. One band is slightly lower than the other. To
>my knowledge, proteolysis of the band (25 kDa) does not
>occur. Is this phenomenon artefact? How can I prevent it?
Is it possible that 25 kDa is present in both phosphorylated and
dephosphoryalted forms, in which case the lower band is phosphoryalted
Lombardi Cancer Center
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