omission mutagenesis

[Elias Fernandez]

would anyone have carried out a mutagenesis where a single residue was 
ommited in the coding gene? 
would it be possible to use a primer with a codon missing and generate 
the mutant by pcr amplification so that the protein has a residue missing?

thanks
elias

--

===============================================================================
 Elias J. Fernandez                 | 
 University of California           |   ## ##     "It's not just an adventure;
 Section of Molecular and           |   (@ (@      it's a job!"
 Cellular Biology                   |      \
 Davis, CA 95616                    |   #####  |
 Tel. No.  : (916)-752-3356         |   ~\_====+
 Fax  No.  : (916)-752-3085
 e-mail    : ejfernandez at ucdavis.edu
==============================================================================