Low copy # reporter?
brad at corona.med.utah.edu
Tue Aug 1 17:22:50 EST 1995
In article <DCn76D.DDs at riker.neoucom.edu>, rjv at uhura.neoucom.edu (Richard
>Does any one know of a reporter vector (luciferase, CAT, and/or B-gal)
>that contains the pBR322 (pMB1) replicon?
Have you considered the pRS series vectors? They are pBR322 based operon
and protein fusion vectors with the ability to move them onto the E. coli
chromosome on a lambda prophage. The reference is: Simons, R.W., Houman,
F. and N. Kleckner. Improved single and multicopy lac-based cloning vectors
for protein and operon fusions. 1987. Gene. 53:85-96.
This system works really well, especially once you get to single copy.
Dr. Simons address (from the Gene paper) is:
University of California, Los Angeles
Department of Microbiology
5304 Life Sciences
Los Angeles, CA 90024
Good Luck, let me know if you have any questions.
Brad Nicholson |"If it worked the first time, it wouldn't be
Department of Pathology | research."...Brad Nicholson
University of Utah | Live from behind the Zion Curtian.
Salt Lake City, UT 84132 |
brad at corona.med.utah.edu |
or: (801)-581-4365 | My opinions are solely my own.
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