Gradients with automated DNA sequencers?
tmurray at nyc.pipeline.com
Wed Aug 2 12:47:49 EST 1995
Why would one wish to use a wedge when such a gel is primarily intended to
compress the short fragment bands in a "manual" gel? It make no sense in a
gel using a "finishing post" type detector.
In bionet.molbio.methds-reagnts HARDIES at THORIN.UTHSCSA.EDU said:
>From: HARDIES at THORIN.UTHSCSA.EDU
>Subject: Gradients with automated DNA sequencers?
>Date: 2 Aug 1995 08:32:06 -0700
>Organization: BIOSCI International Newsgroups for Molecular Biology
>Sender: daemon at net.bio.net
>Message-ID: <950802103414.21c437 at thorin.uthscsa.edu>
>Does anyone use (or know of) wedge gels (or other gradient configurations)
>on an ABI sequencer (or other DNA sequencer with a fixed sensor position)?
>writing a grant proposal about gradient systems in DNA sequencing, and am
>the impression that they aren't applied much to automated sequencers. Am
>Steve Hardies, Assoc. Prof. of Biochem., Univ. of Texas HSC at San Antonio
>Hardies at uthscsa.edu
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