Gradients with automated DNA sequencers?

Tony Murray tmurray at nyc.pipeline.com
Wed Aug 2 12:47:49 EST 1995


Why would one wish to use a wedge when such a gel is primarily intended to
compress the short fragment bands in a "manual" gel? It make no sense in a
gel using a "finishing post" type detector. 
 
Tony 
 
In bionet.molbio.methds-reagnts HARDIES at THORIN.UTHSCSA.EDU said: 
 
 
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>ORIN.UTHSCSA.EDU!HARDIES 
>From: HARDIES at THORIN.UTHSCSA.EDU 
>Newsgroups: bionet.molbio.methds-reagnts 
>Subject: Gradients with automated DNA sequencers? 
>Date: 2 Aug 1995 08:32:06 -0700 
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> 
>Hi Netters: 
> 
>Does anyone use (or know of) wedge gels (or other gradient configurations)
used  
>on an ABI sequencer (or other DNA sequencer with a fixed sensor position)?
 I'm  
>writing a grant proposal about gradient systems in DNA sequencing, and am
under  
>the impression that they aren't applied much to automated sequencers.  Am
I  
>misinformed? 
> 
>Steve Hardies, Assoc. Prof. of Biochem., Univ. of Texas HSC at San Antonio
 
>Hardies at uthscsa.edu 
> 
>For information on our graduate program in Biochemistry:  
>http://bioc02.uthscsa.edu/biochem.html 
-- 
Tony Murray 
Research & Business Development 
Pharmacia Biotech Inc. 
800 Centennial Ave 
Piscataway NJ 08855-1327  
USA 
(908)-457-8392 (tel)  (908)-457-8246 (fax) 



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