RT-PCR and gels

Holger Bohnemeier Bohnemeier at ukbf.fu-berlin.de
Wed Aug 2 06:32:28 EST 1995


In article <3vlv47$h9c at hermes.oc.com>, Gordon Betts <betts at orion.etsu.edu> says:
-shortened-
>
>OK, but in RT I am using either oligo-dT or random primers.  Won't
>this result in ALL mRNA being RT'ed and getting lots of cDNA regardless
>of whether the transcript I'm looking for is there?
>Gordon

You could use the primers you suggested as a controll for your RT-reaction in
total labeling them. Another possibility would be if you use a specific
oligo complementary to the cDNA you are interested in as an RT-primer.


Holger



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