pET vectors, good or bad?

Mike Holloway mike.holloway at stjude.org
Wed Aug 2 21:12:41 EST 1995


In article <199508021441.JAA17718 at mail.utexas.edu>, kbrowning at MAIL.UTEXAS.EDU (Karen Browning) says:

>Also, some people use co-expression of groEL and groES chaparone 
>proteins to get good expression/activity.

Thanks. That's interesting, though in my case it probably wouldn't make 
much difference since I can't even get the plasmid to replicate in JM109.

>If pET does not work for your protein, try something else.  But don't knock 
>a system just because it did not work for your particular protein.

Won't hear a condemnation from me.  It was my first choice, after all.  
When it works, it seems to work beautifully.  But Jeff Kramer's advice 
fits nicely with my own thoughts.  Time to try Pichia yeast.  Nice to 
know I'm not the only one.  

If only I could figure out what the bacteria's problem is.  It might be 
useful information about my protein's function.

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