RNA Extraction (RVCs)
D.J.Glover at bham.ac.uk
Thu Aug 3 11:49:58 EST 1995
The literature states that when using ribonucleoside vanadyl
complexes (RVC) as an RNase inhibitor during RNA extraction, high
concentrations of EDTA and SDS should be avoided as they may cause
dissociation of the complexes and thus their inactivation. Does anyone know
the lowest molar concentration of EDTA that causes dissociation of the
compexes? There is certainly a difference in the colour of the
solution when RVCs are dissolved in buffer containing 10mM and 2mM EDTA.
Thanks in advance.
More information about the Methods