RNA Extraction (RVCs)

[David Glover]

                  The literature states that when using ribonucleoside vanadyl 
complexes (RVC) as an RNase inhibitor during RNA extraction, high 
concentrations of EDTA and SDS should be avoided as they may cause 
dissociation of the complexes and thus their inactivation. Does anyone know 
the lowest molar concentration of EDTA that causes dissociation of the 
compexes? There is certainly a difference in the colour of the 
solution when RVCs are dissolved in buffer containing 10mM and 2mM EDTA.

Thanks in advance.
David Glover.