lidaniel at wiccmail.weizmann.ac.il
Thu Aug 3 09:38:33 EST 1995
R. M. McCormick. "A solid-phase extraction procedure for DNA purification."
Anal Biochem. 181,66-74(1989)
The preparation and use of particulate materials for the removal of proteins
from nucleic acid samples by solid-phase extraction procedures are described.
The solid-phase extraction procedure is analogous to the classical phenol
extraction for DNA purification, with the exception that the phenol is
replaced with insoluble particulate materials that are chemically similar to
phenol and thus function in an analogous manner. These particulate materials
have a very high affinity for proteins and a very low affinity for nucleic
acids. With these materials, it is possible to remove large quantities of
proteins (i.e., tens of milligrams) from minute quantities (submicrogram) of
nucleic acid and quantitatively recover the latter in a biologically active
state. Compared to other procedures that are currently used to purify nucleic
acids, the protocols using these materials offer the advantages of speed,
quantitative DNA recovery, safety, and convenience.
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