Induction by IPTG

[Andy Kravetz]

Stephen Mamber (mamber at synapse.bms.com) wrote:
: In article <bheymann-2407950912360001 at biomac1d.bio.purdue.edu>, bheymann at bragg.bio.purdue.edu (Bernard Heymann) says:
: >
: >I'm interested in the relationship between the concentration of IPTG and
: >the level of induction of proteins from a lac or tac promoter. 

: From personal experience, I would try 1 mM and 2-fold increases or
: decreases from there to bracket your activity.  Further fine-tuning may
: be desirable (like 0.8 mM as an optimum).

I second that. When using IPTG from a 1M stock, I would dilute it down to 
1mM and let induce for 3h or so. Good results.

andy

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Andy Kravetz
Freelance Journalist
St. Louis, Missouri
akravetz at walden.mo.net
http://walden.mo.net/~akravetz/