need help: QUICK & DIRTY RT-PCR

SEHuang sehuang at
Thu Aug 3 13:37:48 EST 1995

Hi all:

Does anybody knows a protocol that allows RT-PCR (of rather rare
messengers) to be carried out directly on CRUDE cell lysates ??
While for genomic PCR this is not a problem, i couldn't find such a "QUICK
& DIRTY" protocol that worked for RT-PCR. I need to directly RT-PCR
messengers out of many very small cell cultures (eg 24 well plates,
non-confluent), and I would like to circumvent the tedious pocedure of RNA
isolation from such small amount of cells. One other advantage of  RT-PCR
or PCR directly from lysates is the smaller sample-to-sample variation
introduced by any further steps of handling.
Thanks for any help and sugestions.

Sui Huang
Surgical Research
Children's Hospital
Boston, MA 02115
FAX: (617) 355-7043
TEL: (617) 355-5010
e-mail: SEHuang at

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