pET vector problems

John Brennand, Tel 4639/5739/6636 John.J.C.Brennand at APVXC1.Pharmaceuticals.ZENECA.tmailuk.sprint.com
Thu Aug 3 11:42:11 EST 1995


We have experienced some problems with commercially available pET vectors. We 
noted that pET21 but not pET11 had a mutation in the lacI gene that would lead 
to an inability to form tetramers.  This may interfere with expression control 
and hence lead to problems with toxic products.

The moral is - sequence the vectors yourself.





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