buffer for DNase treatment?

rey garcia rey.garcia at stonebow.otago.ac.nz
Fri Aug 4 03:03:41 EST 1995


What buffer should I use for treating RNA samples with DNAse I, prior to
RT-PCR? How long should the incubation be? Is it necessary to phenol
extract after incubation or should inactivation at 70-80 degrees for 15
minutes suffice? Any useful info will be greatly appreciated. Please
e-mail to:

rey.garcia at stonebow.otago.ac.nz






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