help for direct sequencing!!

Lygia da Veiga Pereira lpereira at spider.usp.br
Fri Aug 4 10:12:52 EST 1995

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I need help for direct sequencing of PCR products. I am currently using a 
NEB kit CycleSequence, but its doesn't always work, and it seems to use 
too much S35 (2ul/reaction!).
	What are people currently using?

	Lygia(lpereira at usp.br)

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