help needed with southern

Sergey V. Orlov Serge at bio.iem.ras.spb.ru
Fri Aug 4 23:51:46 EST 1995


Hi, Paul.

The volume of prehybridization solution don't important
( it is necessary that your membrane completely was soaked
with fluid ). Thus, you can use the large amount of this
( I use 25 ml per 100 sq. cm ). While, the volume of
hybridization mix should be minimum ( it allows to achieve
a maximum concentration of your labeling  probe ). Generally,
2 ml per 100 sq. cm  are used but small filters may be request
more amounts.
I hope, this helps.

Sincerely, Serge Orlov



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