Help recovering 200bp DNA fragment

(Dave Johnston) daj at mailserver.nhm.ac.uk
Mon Aug 7 02:17:34 EST 1995


arshinee,
Try using linear polyacrylamide as a carrier in the precipitation. It is 
excellent for recovery of dilute and/or small size fragments. I have been 
able to ptt down to apoprox 4ng DNA without apparent loss using this. See 
paper by Gaillard and Strauss, Nuc. Acids. Res. 18(2) for details (sorry, I 
don't have the page number).
DAJ

David A. Johnston
Research Fellow,
Dept of Zoology, The Natural History Museum, Cromwell Road,
South Kensington, London SW7 5BD. England
(tel 071 9389297, fax 071 9388754, email daj at nhm.ac.uk)



More information about the Methods mailing list