murrayb at dc37a.nci.nih.gov
Mon Aug 7 15:16:57 EST 1995
[Ownership of message below has become lost]
>>> Yesterday I found that the only clone had died!!! (dried out in incubator;
>>> technician forget to add water). The month of hard work trashed.
>>> It kills me, I need rest. I want vacation.
[Helpful advice for plasmid rescue deleted]
Ah, this assumes that it is a *plasmid* clone! What if it was a cell clone?
(yes, I know that this newsgroup is molbio but....). Having once lost
monoclonal antibody secreting hybridoma lines through liquid nitrogen
freezer failure I can sympathise on that front. I also read an article
some time ago that it was possible to harvest the genomic DNA from such
dead hybridoma lines rescue the clone. I don't know if this was intended
for antibody production in bacteria/baculo etc. or if the genes were moved
to another cell but the whole idea sounded neat.
Hopefully it was a nice easy plasmid that could be rescued...
bernard at elsie.nci.nih.gov
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