tnakane at GIPAC.SHINSHU-U.AC.JP
Mon Aug 7 21:29:28 EST 1995
We are currently trying to clone a G protein-coupled receptor by panning method.
We constructed a rat brain cDNA library (pME18S vector; SR-alpha promoter)
and transfected the library to COS-7 cells by DEAE dextran method. After
72 hrs, we could not detach cells from dishes by the treatment with PBS and
5 mM EDTA at 37 C for 30 min. The untransfected cells were easily detached
by the treatment. Any advice would be much appreciated.
More information about the Methods