bates at cismsun.univ-lyon.fr
Mon Aug 7 10:07:23 EST 1995
pnh at fcsparc6.ncifcrf.gov (Paul N Hengen) wrote:
>> Yesterday I found that the only clone had died!!! (dried out in incubator;
>> technician forget to add water). The month of hard work trashed.
>> It kills me, I need rest. I want vacation.
>Wait!!! Don't throw out that dead culture! ......
PCR also works. I know, the same thing happened to me except it was me that forgot the clone. (No one else to blame...). Scrape a bit of the dead culture into water and PCR with a 5 min denaturation at the begining. This way you can also make recloning easier by choosing your oligos.
Hope this helps. Liz.
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