Transfection of B cell-lines
cocea at citi2.fr
Mon Aug 7 06:39:40 EST 1995
r.p.yeo at reading.ac.uk wrote:
> I am trying to transfect Daudi and Raji cell-lines with a number of
> expression vectors with either HCMV or RSV LTR promotors with very little
> success. Is there anybody out there with experience in transfecting these
> lines either transiently or stable and could they email me a procotol.
> Thanking you in advance.
> Paul Yeo
> The University of Reading
> Whiteknights, PO BOX 228
> READING, RG6 6AJ
> Email r.p.yeo at reading.ac.uk
Hi- I have not tried to transfect either Daudi or Raji cells but I
transfect quite often 230/238 mouse pre-B cells with DEAE-dextran and
it works fine. If you want the protocol just let me know and I
shall send it to you. I'm at cocea at citi2.fr.
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