*** TRizol LS for Tissue RNA ***

HOFSTRA ANGELA 905-822-3520 Hofstra at aa.wl.com
Tue Aug 8 12:47:38 EST 1995

>I want to use Trizol LS (BRL) for isolating RNA from tissue samples.  I've
>used it successfully in several other RNA isolations from cell culture,
>blood, etc.
>The tissue protocol calls for using a "tissuemizer" or some other tissue
>homogenization machine.  Has anyone used Trizol for tissue RNA isolation
>without using a homogenizer machine?  Can you just cut tissue up and
>vortex?  I don't have enough tissue to see if that'll work.  Thankyou.

We use Trizol for harvesting RNA from liver tissue and cultured liver cells. 
For the first we do homogenize.  For the second we force the cells in Trizol
through a 22-26g needle.  It works very well.
>Brad Schonhoft                      bschonho at magnus.acs.ohio-state.edu
>Dept.of Molecular Genetics          Ph. 614-292-2640
>484 W. 12the Ave, Rm 226
>Columbus, Oh 43201

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