*** TRizol LS for Tissue RNA ***

[HOFSTRA ANGELA 905-822-3520]

>I want to use Trizol LS (BRL) for isolating RNA from tissue samples.  I've
>used it successfully in several other RNA isolations from cell culture,
>blood, etc.
>The tissue protocol calls for using a "tissuemizer" or some other tissue
>homogenization machine.  Has anyone used Trizol for tissue RNA isolation
>without using a homogenizer machine?  Can you just cut tissue up and
>vortex?  I don't have enough tissue to see if that'll work.  Thankyou.
>

We use Trizol for harvesting RNA from liver tissue and cultured liver cells. 
For the first we do homogenize.  For the second we force the cells in Trizol
through a 22-26g needle.  It works very well.
>-- 
>Brad Schonhoft                      bschonho at magnus.acs.ohio-state.edu
>Dept.of Molecular Genetics          Ph. 614-292-2640
>484 W. 12the Ave, Rm 226
>Columbus, Oh 43201