Transfection of B cell-lines

Mike Smith smith-m at
Tue Aug 8 10:51:12 EST 1995

Laurentiu COCEA <cocea at> wrote:
>r.p.yeo at wrote:
>>    I am trying to transfect Daudi and Raji cell-lines with a number of
>> expression vectors with either HCMV or RSV LTR promotors with very little
>> success.  Is there anybody out there with experience in transfecting these
>> lines either transiently or stable and could they email me a procotol. 
>> Thanking you in advance.
>> Paul Yeo
>> AMS,
>> The University of Reading
>> Whiteknights, PO BOX 228

I've had good success with Raji (haven't tried Daudi) using 
electroporation.  Using the BioRad or BTX machines.  
500V/220 ufd capacitance in a 4 mm cuvette.  Used 20ug DNA 
in 200ul of cells in RPMI1640+10%FBS.  If you want any more 
details email me.  Good Luck

Mike Smith, Ph.D.
Univ. of Colorado HSC

More information about the Methods mailing list