Transfection of B cell-lines
smith-m at oberon.hsc.colorado.edu
Tue Aug 8 10:51:12 EST 1995
Laurentiu COCEA <cocea at citi2.fr> wrote:
>r.p.yeo at reading.ac.uk wrote:
>> I am trying to transfect Daudi and Raji cell-lines with a number of
>> expression vectors with either HCMV or RSV LTR promotors with very little
>> success. Is there anybody out there with experience in transfecting these
>> lines either transiently or stable and could they email me a procotol.
>> Thanking you in advance.
>> Paul Yeo
>> The University of Reading
>> Whiteknights, PO BOX 228
>> READING, RG6 6AJ
I've had good success with Raji (haven't tried Daudi) using
electroporation. Using the BioRad or BTX machines.
500V/220 ufd capacitance in a 4 mm cuvette. Used 20ug DNA
in 200ul of cells in RPMI1640+10%FBS. If you want any more
details email me. Good Luck
Mike Smith, Ph.D.
Univ. of Colorado HSC
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