beall.3 at OSU.EDU
Tue Aug 8 09:11:35 EST 1995
In article <9508080226.AA12954 at gipac.shinshu-u.ac.jp>,
tnakane at GIPAC.SHINSHU-U.AC.JP (tokio nakane) wrote:
> We are currently trying to clone a G protein-coupled receptor by panning
> We constructed a rat brain cDNA library (pME18S vector; SR-alpha promoter)
> and transfected the library to COS-7 cells by DEAE dextran method. After
> 72 hrs, we could not detach cells from dishes by the treatment with PBS and
> 5 mM EDTA at 37 C for 30 min. The untransfected cells were easily detached
> by the treatment. Any advice would be much appreciated.
You might try 293 cells (human embryonic kidney). They are generally
easier to detach from plates.
Ohio State Neurobiotechnology Center
beall.3 at osu.edu
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