Purification of egg yolk antibodies

Frank Neumann Frank.Neumann at uni-konstanz.de
Tue Aug 8 06:15:34 EST 1995


Trying to get antibodies against conserved protein. Initial results with
modified Gassmann et al. FASEB 90 protocol quite promissing, but antibody
preparations seem sensitive to repeated thawing/freezing (-70) cycles. How
to store them safely? Another problem: high background sometimes. Does
anybody have experiences how to further purify them (protocol for antigen
affinity column- IgY binding/elution, protein A equivalent)? Currently
testing gamma Yolk purification kit from Pharmacia Biotech. Too expensive
for our group to be used in general. Anything known about the composition
of separation reagent 1, buffer 2, and separation reagent 3?
Thanks Frank

*******************************************
Frank Neumann
Inst. of Immunology
University of Constance
Postfach 5560 M 660

78434 Konstanz
Germany

Phone: +49-7531-88-3007
Fax:     +49-7531-88-3102
e-mail: Frank.Neumann at uni-konstanz.de
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