PCR Artifact?

Steven Sullivan sullivan at gwis2.circ.gwu.edu
Tue Aug 8 18:48:02 EST 1995


Gordon Betts (betts at orion.etsu.edu) wrote:
: I just ran a gel of my PCR product and I'm getting a faint smear
: at the bottom at around 100 bp size. Hardly the 2000 bp I want.
: Is this some kind of artifact or am I getting a short cDNA?
: Thanks,
: Gordon


how big are your primers?  is the smear really 100 bp or is it smaller?
smears at the bottom are usually either primer-dimers or unincorporated 
nucleotides.





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