direct PCR from bacterial colonies.

bch127 bch127
Tue Aug 8 11:57:52 EST 1995


Does anyone have a method for PCr amplification of a plasmid from bacteria
scraped off a plate into a 96-well plate as an alternative to minipreps, i.e
how do you make sure that the bacteria lyse ? is 5 minutes at 95 C enough or do
you have to do anything else ? I'm sure I heard someone talking about this once
but I cant remember where.
		Neil Taylor bch127 at abdn.ac.uk




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