Library screening alternatives?
spagnol at galactica.it
Tue Aug 8 23:20:10 EST 1995
In article <3vp470$775 at cronkite.seas.gwu.edu> Steven Sullivan,
sullivan at gwis2.circ.gwu.edu writes:
> That takes time, especially if you like crowded plates (50,000 plaques
> per 150 mm plate), = : which requires secondary and tertiary
> purifications, and then--DREAD!--growing up a good-sized high-titer
> stock for DNA purifi= : cation. Remember, to get 1 !g of 2 kb insert
> need 20 !g of pure recombinant DNA. I find it very easy and convenient
> pop out= : the inserts using PCR. New England Biolabs sells forward
> reverse primers for lambda gt11 flanking the Eco RI site (24-mers). = :
> They work easily, especially if the insert is not cut by Eco RI,
> a very quick shuttle from phage->PCR product->EcoRI-cut PC= : R
You may have a look in one of the last number of science. They (I do not
remember which company) advertise a fastest screening method, which I
never tried, however.
No affiliations etc.
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