Library screening alternatives?

Giorgio Spagnol spagnol at galactica.it
Tue Aug 8 23:20:10 EST 1995


In article <3vp470$775 at cronkite.seas.gwu.edu> Steven Sullivan,
sullivan at gwis2.circ.gwu.edu writes:
>  That takes time, especially if you like crowded plates (50,000 plaques
> per 150 mm plate), = : which requires secondary and tertiary
> purifications, and then--DREAD!--growing up a good-sized high-titer
phage
> stock for DNA purifi= : cation.  Remember, to get 1 !g of 2 kb insert
you
> need 20 !g of pure recombinant DNA.  I find it very easy and convenient
to
> pop out= :  the inserts using PCR.  New England Biolabs sells forward
and
> reverse primers for lambda gt11 flanking the Eco RI site (24-mers).  = :
> They work easily, especially if the insert is not cut by Eco RI,
allowing
> a very quick shuttle from phage->PCR product->EcoRI-cut PC= : R
> product->plasmid. 

You may have a look in one of the last number of science. They (I do not
remember which company) advertise a fastest screening method, which I
never tried, however.
Good look.
No affiliations etc.
Giorgio
>



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